Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Bicine Buffer (0.5 M, pH 8.5) - 250ml
Bicine Buffer (0 5 M pH 8 5) - 250ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HEPES Buffer (0.5 M, pH 7.0) - 250ml
HEPES Buffer (0 5 M pH 7 0) - 250ml (For Research Use and Further Manufacturing Only)
Teknova 1M Tris-HCl, pH 7.5. 500ml Sterile. 4 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova Tris Glycerol Buffer pH 8.0. 1000mL, Endotoxin Tested, Sterile 2 pack.
20mM Tris,25mM Sodium Chloride,2.5% Glycerol
Teknova CTAB Extraction Solution. 250mL. 4 Pack.
2.0% CTAB, 100mM Tris-HCl, pH 8.0,20mM EDTA, pH 8.0, 1.4M Sodium Chloride
Teknova 2M Tris-HCl, pH 7.5 Solution. 1000mL, Sterile. 2 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova 1X Dulbeccos Phosphate Buffered Saline with 0.5% BSA. 1000mL, Sterile. 2 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (20X) - 1L
Phosphate Buffered Saline PBS (20X) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.4) - 1L
Tris-HCl Buffer (1 M pH 7 4) - 1L (For Research Use and Further Manufacturing Only)
Teknova 20X PBS with 1% Tween-20. 500mL, Sterile. 8 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Teknova 1X PBS Solution, pH 7.5. 2L, Sterile 2 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.0) - 1L
Tris-HCl Buffer (1 M pH 7 0) - 1L (For Research Use and Further Manufacturing Only)
Teknova SM Buffer. 1000mL, Sterile 2 pack.
100mM Sodium Chloride, 8mM Magnesium Sulfate,0.01% Gelatin, 50mM Tris-HCl
Teknova 0.45% Saline Solution, Sterile. 500mL 4 pack.
Sodium chloride is an essential ingredient in the lab. Not only does it help regulate the pH of solutions, but it also prevents growth of bacteria and other contaminants. In addition, salt is a powerful desiccant, meaning that it can help to dry out wet samples. As a result, salt is often used in a wide variety of laboratory procedures.
